Sy. Lee et al., CD30 TNF RECEPTOR-ASSOCIATED FACTOR INTERACTION - NF-KAPPA-B ACTIVATION AND BINDING-SPECIFICITY/, Proceedings of the National Academy of Sciences of the United Statesof America, 93(18), 1996, pp. 9699-9703
CD30 is a member of the tumor necrosis factor (TNF) receptor superfami
ly, CD30 is expressed on normal activated lymphocytes, on several vira
lly transformed T- or B-cell lines and on neoplastic cells of Hodgkin'
s lymphoma, The interaction of CD30 with its ligand induces pleiotropi
c effects on cells resulting in proliferation, differentiation, or dea
th, The CD30 cytoplasmic tail interacts with TNF receptor-associated f
actors (TRAFs), which have been shown to transduce signals mediated by
TNF-R2 and CD40, We demonstrate here that TRAF2 also plays an importa
nt role in CD30-induced NF-kappa B activation, We also show that TRAF2
-mediated activation of NF-kappa B plays a role in the activation of H
IV transcription induced by CD30 cross-linking, Detailed site-directed
mutagenesis of the CD30 cytoplasmic tail reveals that there are two i
ndependent binding sites for TRAF, each interacting with a different d
omain of TRAF, Furthermore, we localized the TRAF-C binding site in CD
30 to a 5-7 amino acid stretch.