REVERSED-PHASE HPLC OF PEPTIDES WITH TETRAPHENYLPORPHYRIN-BASED STATIONARY-PHASE AND POTENTIOMETRIC DETECTION WITH A COPPER ELECTRODE

The retention of selected amino acids and simple peptides has been inv estigated using columns with stationary phases with immobilized [5-(p- carboxyphenyl)-10,15,20-tri-phenyl]porphyrin and zinc(II) ion as coord ination center. The use of potentiometry with a metallic copper electr ode provides an especially sensitive detection of peptides containing histidine. The retention mechanism includes both interactions between the aromatic moieties of the solute molecule and immobilized metallopo rphyrin and a complexation interaction between solutes and the metalli c center of the metalloporphyrin.