THE MORPHOLOGY, CYTOLOGY, AND C-BANDED KARYOTYPES OF BRASSICA-CAMPESTRIS, BRASSICA-OLERACEA, AND BRASSICA-NAPUS PLANTS REGENERATED FROM PROTOPLASTS

The behaviour of Brassica campestris (2n = 20, AA), B. oleracea (2n = 18, CC), and B. napus (2n = 38, AACC) were studied during a tissue-cul turing process. Hypocotyl-protoplasts were cultivated into calli from which new plants were regenerated. The regenerated plants were compare d, and mitotic root-tip cells were C-banded and karyotyped. A majority of the plants were tetraploid. The meioses were studied in the PMCs. A number of abberations were observed, mainly due to faulty spindle fu nction. There was a difference between the three species in that B. ca mpestris performed the most poorly with many fewer regenerated plants. These plants were more morphologically disturbed and had more problem s during pollen production than B. oleracea and B. napus plants.