COMPLETE H-1 AND C-13 RESONANCE ASSIGNMENTS OF GLYCOSIDIC CONJUGATES OF HYODEOXYCHOLIC ACID BY 2-DIMENSIONAL NMR TECHNIQUES

H-1 and C-13 NMR signals were completely assigned for six 3 alpha- and 6 alpha-O-beta-D-glycosidic conjugates (glucosides, glucuronides and N-acetylglucosaminides) of hyodeoxycholic (3 alpha,6 alpha-dihydroxy-5 beta-cholanoic) acid as their methyl ester-acetate derivatives (three being 3-glycosides and three corresponding 6-analogues), The signal a ssignments were performed using conventional 1D NMR and several homonu clear and heteronuclear shift-correlated 2D NMR techniques (H-1-H-1 CO SY, H-1-H-1 NOESY, H-1-H-1 HOHAHA, H-1-C-13 HETCOR, long-range H-1-C-1 3 HETCOR), The H-1 and C-13 signals serving to characterize each of th e bile acid glycosides were identified and used in the structural eluc idation of these types of biologically important compounds.