K. Brand et al., HYPERTHERMIA DECREASES CYTOKINE-MEDIATED ADHESION MOLECULE EXPRESSIONON HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS, International journal of hyperthermia, 12(4), 1996, pp. 527-538
Citations number
35
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging",Oncology
Although hyperthermia has been used as an effective cancer treatment m
odality, its effects on metastasis of tumour cells are not clear. Sinc
e adhesion molecules play a key role in metastasis: we evaluated how t
he expression of adhesion molecules is influenced by hyperthermia. Hum
an umbilical vein endothelial cells were incubated in vitro for 1 h. a
t 39, 42, 43 and 44 degrees C with and without addition of tumour-necr
osis factor (TNF) or interferon-gamma (IFN-gamma) and the expression o
f endothelial cell leukocyte adhesion molecule-1 (ELAM-1), intercellul
ar adhesion molecule-1 (ICAM-1) and major histocompatibility complex (
MHC) class-II molecule was measured. Expression of MHC class-II molecu
les and expression of unstimulated constituent ICAM-1's was not reduce
d by heat treatment. In contrast, expression of cytokine-induced ELAM-
1's and ICAM-1's was significantly lower after heat treatment. The adh
esion to HUVEC in vitro of HL-60 leukemia cells, which express sialyl-
Lewis-x antigen as a ligand to ELAM-1, was diminished after incubation
at 42 degrees C and totally lost after treatment at 44 degrees C. Thi
s suggests that any decrease in metastasis formation after heat treatm
ent, which is occasionally observed, could be due to a reduced action
of TNF or related cytokines on adhesion molecule induction and subsequ
ent membrane expression by the endothelial cell. A possible underlying
mechanism involved is a heat-induced alteration or blockage of the bi
osynthetic pathways required for synthesis of ELAM-1 and ICAM-1 protei
ns.