DETERMINATION OF RESIDUES IMPORTANT IN HORMONE-BINDING TO THE EXTRACELLULAR DOMAIN OF THE LUTEINIZING-HORMONE CHORIONIC-GONADOTROPIN RECEPTOR BY SITE-DIRECTED MUTAGENESIS AND MODELING
N. Bhowmick et al., DETERMINATION OF RESIDUES IMPORTANT IN HORMONE-BINDING TO THE EXTRACELLULAR DOMAIN OF THE LUTEINIZING-HORMONE CHORIONIC-GONADOTROPIN RECEPTOR BY SITE-DIRECTED MUTAGENESIS AND MODELING, Molecular endocrinology, 10(9), 1996, pp. 1147-1159
The LH/CG receptor (LH/CG-R) belongs to the family of glycoprotein hor
mone G protein-coupled receptors. The extracellular domain of LH/CG-R
is associated with high ligand-binding affinity and contains leucine-r
ich repeats (LRRs). With the goal of identifying essential amino acid
residues involved in ligand binding, we replaced several conserved ion
izable residues in the rat LH/CG-R with ones of opposite charge. The e
xpression of these mutants was assessed by binding studies and Western
blots after COS-7 cells were transiently transfected with wild type a
nd mutant receptor cDNAs. The charge inversion of each of Lys(40) Lys(
104), Asp(118), Glu(132), and Asp(135) with Asp or LYS resulted in no
detectable human CG binding in intact or solubilized cells; as control
, a Lys(40)-->Arg replacement yielded a mutant with characteristics of
the wild type receptor. Western analysis showed that the Lys(40)-->Ar
g mutant expressed at a level comparable to that of wild type receptor
and, like wild type, exhibited a predominant immunoreactive mature fo
rm of LH/CG-R. Each of the five charge inversion mutants expressed at
a lower level than wild type as assessed by immunoreactivity, and the
levels of the Lys(40)-->Asp and Glu(132)-->Lys mutants were particular
ly low. The ratio of the mature to immature form of the receptor was h
igh, i.e, like that of wild type, for the Glu(132)-->Lys and Asp(135)-
->Lys replacements; the three other charge inversion mutants exhibited
less mature than immature forms of the receptor. To aid in interpreti
ng these results, we developed a model incorporating residues 27-235 o
f the extracellular domain of the rat LH/CG-R based on the crystal str
ucture of the porcine ribonuclease inhibitor. Sequence homology and al
ignment revealed nine LRRs, with flanking cysteine clusters as found i
n a number of LRR proteins. Our model suggested that the Lys replaceme
nts of Glu(132) and Asp(135), i.e. those mutants that formed mature re
ceptors, would disrupt the regional negative charge of the receptor. W
e propose that these residues are either directly involved in hormone
binding or indirectly by disruption of the charge of an important bind
ing surface.