ACTIVATION OF ANATOXIN B-1 BY MOUSE CYP2A ENZYMES AND CYTOTOXICITY INRECOMBINANT YEAST-CELLS

The ability of three highly homologous mouse liver CYP2A enzymes to ac tivate aflatoxin B-1 was studied by expressing them in recombinant AH2 2 Saccharomyces cerevisiae yeast cells. The reconstituted monooxygenas e complex with CYP2A5 purified from yeast cell microsomes produced epo xide at a rate of 17.2 nmol/min per nmol P450 in the presence of 50 mu M aflatoxin B-1 while CYP2A4 had about 10% and P4507 alpha only 1.5% of this activity. However, K-m values were 530 and 10 mu M and V-max v alues 12.5 and 14.3 nmol/min per nmol P450 for CYP2A4 and CYP2A5, resp ectively. When recombinant yeast cells were exposed to aflatoxin B-1 L C(50) concentrations were 7.5 +/- 5.5 mu M for CYP2A4, 0.45 +/- 0.10 m u M for CYP2A5 and > 320 mu M for P4507 alpha expressing yeast cells. Aflatoxin B-1-DNA adduct levels in the same yeast cells were 50, 890 p mol/mg DNA and below detection limit when 3.0 mu M aflatoxin B-1 was u sed in the incubation mixture. Coumarin an inhibitor for CYP2A4 and a substrate for CYP2A5 diminished the toxicity of aflatoxin B-1 in a dos e-dependent manner for these recombinant yeast cells. These data demon strate that (1) highly homologous mouse CYP2A enzymes activate aflator dn B-1 in a different manner and (2) that recombinant yeast cells expr essing mammalian CYP enzymes are a useful and inexpensive system to te st the role of different enzymes in aflatoxin B-1 toxicity. The data a lso indicate that mouse CYP2A5 and its counterpart in other species co uld have a significant role in aflatoxin B-1 toxicity in organs where it is expressed at high levels.