G. Tonello et al., CHARACTERIZATION AND QUANTITATION OF THE ACTIVE POLYNUCLEOTIDE FRACTION (PDRN) FROM HUMAN PLACENTA, A TISSUE-REPAIR STIMULATING AGENT, Journal of pharmaceutical and biomedical analysis, 14(11), 1996, pp. 1555-1560
The polydeoxyribonucleotide (PDRN) fraction is an extract which forms
the active component in a new formulation of the drug Placentex (a tis
sue repair stimulating agent) obtained from human placenta through an
original proprietory extraction method. From a comparison of the UV, N
MR and IR spectra of this fraction (before and after nuclease treatmen
t) with that of a similar standard (Sigma D1501), it was shown that th
e active substances in the PDRN fraction mainly consist of a mixture o
f DNA fragments. By gel electrophoresis, the molecular weights of the
DNA fragments were shown to range from 50 to 2000 base pairs. Finally,
an HPLC method is described, based on an anion-exchange material capa
ble of determining the amount of PDRN in different batches of the extr
act, which varied from 80 to 90%.