AN AUTOMATED SAMPLE PREPARATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF MK-591, A NOVEL LEUKOTRIENE BIOSYNTHESIS INHIBITOR, IN HUMAN PLASMA
C. Lin et al., AN AUTOMATED SAMPLE PREPARATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF MK-591, A NOVEL LEUKOTRIENE BIOSYNTHESIS INHIBITOR, IN HUMAN PLASMA, Journal of pharmaceutical and biomedical analysis, 14(11), 1996, pp. 1601-1608
A high performance liquid chromatography assay utilizing an automated
sample preparation procedure for the determination of a novel leukotri
ene biosynthesis inhibitor, (MK-591), in human plasma has been develop
ed. After aliquoting plasma samples and adding internal standard manua
lly, the BenchMate (TM) Workstation executed protein precipitation and
solid-phase extraction. Following evaporation to dryness, the residue
was reconstituted and chromatographed isocratically on a cyano-phase
analytical column. MK-591 and the internal standard were separated fro
m each other and from endogenous plasma substances and detected with a
n absorbance detector. The assay has been validated in the concentrati
on range 10-1000 ng ml(-1) and has the sensitivity and specificity nec
essary to quantify plasma concentrations from several clinical studies
.