AN AUTOMATED SAMPLE PREPARATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF MK-591, A NOVEL LEUKOTRIENE BIOSYNTHESIS INHIBITOR, IN HUMAN PLASMA

A high performance liquid chromatography assay utilizing an automated sample preparation procedure for the determination of a novel leukotri ene biosynthesis inhibitor, (MK-591), in human plasma has been develop ed. After aliquoting plasma samples and adding internal standard manua lly, the BenchMate (TM) Workstation executed protein precipitation and solid-phase extraction. Following evaporation to dryness, the residue was reconstituted and chromatographed isocratically on a cyano-phase analytical column. MK-591 and the internal standard were separated fro m each other and from endogenous plasma substances and detected with a n absorbance detector. The assay has been validated in the concentrati on range 10-1000 ng ml(-1) and has the sensitivity and specificity nec essary to quantify plasma concentrations from several clinical studies .