COMPARATIVE-STUDY ON 4 ENZYME-LINKED IMMUNOSORBENT ASSAYS AND A COCULTIVATION ASSAY FOR THE DETECTION OF ANTIGENS ASSOCIATED WITH THE BOVINE VIRAL DIARRHEA VIRUS IN PERSISTENTLY INFECTED CATTLE

Authors
BRINKHOF J ZIMMER G WESTENBRINK F
Citation
J. Brinkhof et al., COMPARATIVE-STUDY ON 4 ENZYME-LINKED IMMUNOSORBENT ASSAYS AND A COCULTIVATION ASSAY FOR THE DETECTION OF ANTIGENS ASSOCIATED WITH THE BOVINE VIRAL DIARRHEA VIRUS IN PERSISTENTLY INFECTED CATTLE, Veterinary microbiology, 50(1-2), 1996, pp. 1-6
Citations number
14
Categorie Soggetti
Microbiology,"Veterinary Sciences
Journal title
Veterinary microbiology → ACNP
ISSN journal
03781135
Volume
50
Issue
1-2
Year of publication
1996
Pages
1 - 6
Database
ISI
SICI code
0378-1135(1996)50:1-2<1:CO4EIA>2.0.ZU;2-H
Abstract
Four commercially available ELISAs for detection of antigens associate d with the bovine viral diarrhoea virus in persistently infected cattl e have been compared. The tests are equally specific (100%) and the se nsitivity of three ELISAs is comparable with that of a conventional co cultivation assay. Performing ELISA on samples from young animals that received colostrum may yield false negative results because of interf erence of maternal antibodies in the tests. It is concluded that ELISA can be a valuable tool in eradications programs when large numbers of cattle are to be monitored.