SPECIFIC SEPARATION OF ANIMAL-CELLS USING AQUEOUS 2-PHASE SYSTEMS

An aqueous two-phase system composed of polyethylene glycol, dextran, and a functional ligand carrier was developed which enabled the specif ic separation of animal cells. In the presence of more than 0.042 M Na Cl, almost all the cells examined were distributed in the bottom phase and the interphase, whereas the ligand carrier (Eudragit) was distrib uted in the top phase. An antigen was conjugated to Eudragit and the l igand-conjugated carrier was added to polyethyleneglycol 8000-dextran T500 aqueous two-phase systems. Using the systems, hybridoma cells, wh ich produce antibody against the conjugated antigen, could be separate d to the top phase from the parental NS-1 cells. This separation proce dure may be useful for the establishment of a hybridoma cell line.