J. Wubert et al., INHIBITORS OF CHLOROPHYLL BIOSYNTHESIS FROM BULBS OF GLADIOLUS SPP, Zeitschrift fur Naturforschung. C, A journal of biosciences, 51(7-8), 1996, pp. 548-557
Compounds extracted with methanol from the bulbs of Gladiolus spp. wer
e tested for inhibition of Chi accumulation in greening cress (Lepidiu
m sativum L.) seedlings. For the biotest, cress seeds were imbibed wit
h water for 8 h and then incubated in the dark with the test solution
for 40 h. Chlorophyll accumulation was determined at the end of the su
bsequent irradiation period of 8 h. Compounds active in this biotest w
ere purified by several chromatographic steps and identified by thin-l
ayer chromatography with staining reactions and by gas chromatography/
mass spectrometry. The following compounds were identified and their c
oncentration for 50% inhibition of Chi accumulation determined: sucros
e (75 mM) plus glucose (30 mM), 3-hydroxybenzoic acid methylester (0.4
9 mM) and 5-hydroxymethylfurfural (6.7 mM). The inhibitory action was
compared with several substituted derivatives of the natural compounds
.