INTERACTION OF ESCHERICHIA-COLI RIBONUCLEASE-H WITH HYBRID DUPLEXES CONTAINING 2'-DEOXYXYLOTHYMIDINE, 2'-DEOXY-2'FLUOROURIDINE OR ALPHA-THYMIDINE

Authors
ALEKSEEV YI KRYNETSKAYA NF TASHLYTSKY VN ORETSKAYA TS DOLINNAYA NG MORVAN F RAYNER B MALVY C SHABAROVA ZA
Citation
Yi. Alekseev et al., INTERACTION OF ESCHERICHIA-COLI RIBONUCLEASE-H WITH HYBRID DUPLEXES CONTAINING 2'-DEOXYXYLOTHYMIDINE, 2'-DEOXY-2'FLUOROURIDINE OR ALPHA-THYMIDINE, Nucleosides & nucleotides, 15(9), 1996, pp. 1545-1558
Citations number
29
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
15
Issue
9
Year of publication
1996
Pages
1545 - 1558
Database
ISI
SICI code
0732-8311(1996)15:9<1545:IOERWH>2.0.ZU;2-D
Abstract
Oligothymidilates with repeating inserts of 1-(beta-D-2'-deoxy-2'-fluo ropentafuranosyl) U),1-(beta-D-2'-deoxy-threo-pentafuranosyl)thymine ( xT)an d alpha-oligodeoxyribonucleotide alpha(G(2)T(12)G(2)) were synth esized. Hybrid duplexes were obtained to study the physico-chemical pr operties (melting curves, CD-spectra) and the interaction with E. coli ribonuclease H. It was found that the modified hybrid duplex prA(18)/ TT(fUTT)(6) did not bind the enzyme, the modified hybrid duplex prA(18 )/(xTTT)(6) inhibited oligoriboadenylate cleavage by RNase H in hybrid duplexes prA(20)/(G(2)T(20)) and prA(14)/T-16 as well as the modified hybrid duplex prA(14)/alpha(G(2)T(12)G(2)).