DETECTION OF MAREKS-DISEASE VIRUS SEROTYPE-1 (MDV1) GLYCOPROTEIN-D INMDV1-INFECTED CHICK-EMBRYO FIBROBLASTS

Chick embryo fibroblasts (CEFs) infected with three strains of Marek's disease virus serotype 1 (MDV1), GA, Md5 and JM, were subjected to in direct immunofluorescence assay with monoclonal antibodies (MAbs) agai nst MDV1 homolog of glycoprotein D (MDV1 gD) of herpes simplex virus. By the MAbs, a number of MDV1 go-positive cells were detected in CEFs infected with GA, whereas only a few and no positive cells were detect ed in CEFs infected with Md5 and JM, respectively. The MDV1 go in GA-i nfected CEFs was recognized as the band of 64 kDa in immunoblot analys is using one of the MAbs. This is the first report that the MDV1 go wa s detected in MDV1-infected cell cultures.