CLONING OF SUCRASE OPERON WITH MINI-MU AND PLASMID-MEDIATED METABOLISM OF SUCROSE

Authors
GRONES J MACOR M BILSKA V
Citation
J. Grones et al., CLONING OF SUCRASE OPERON WITH MINI-MU AND PLASMID-MEDIATED METABOLISM OF SUCROSE, Folia microbiologica, 41(4), 1996, pp. 315-319
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Folia microbiologica → ACNP
ISSN journal
00155632
Volume
41
Issue
4
Year of publication
1996
Pages
315 - 319
Database
ISI
SICI code
0015-5632(1996)41:4<315:COSOWM>2.0.ZU;2-M
Abstract
The in vivo cloning system based on mini-Mu derivatives was used for c loning the sucrase operon. We constructed several recombinant plasmids pJT21, pMM2324, pMM2325 with a complete sucrase operon. Two strains o f Escherichia coli containing this plasmid replicon were able to grow on different concentrations of sucrose. The stability of recombinant p lasmids was determined after cultivation under nonselective conditions . The stability after 5-d cultivation was higher than 75%.