Jg. Prudhomme et al., STUDIES OF PLASMODIUM-FALCIPARUM CYTOADHERENCE USING IMMORTALIZED HUMAN BRAIN CAPILLARY ENDOTHELIAL-CELLS, International journal for parasitology, 26(6), 1996, pp. 647-655
The cytoadherence of Plasmodium falciparum-infected erythrocytes was s
tudied using immortalized human brain capillary endothelial cells. The
immortalized cells, denoted as BB19, derived from the human brain end
othelium, were transformed with the E6E7 genes of human papilloma viru
s and retained their endothelial nature, i.e. tubule formation occurre
d with Matrigel as a substratum and the cells stained positive for Fac
tor VIII-related antigen, or vonWillebrand's factor. Surface expressio
n of ICAM-1, VCAM, E-selectin, and CD36 was demonstrated by immunofluo
rescence staining with monoclonal antibodies to these Ligands. Exposur
e to cytokines (TNF, IFN gamma, IL-1 alpha, and IL-6) and lipopolysacc
haride resulted in an increase in expression of ICAM-1, VCAM, E-select
in, and CD36. The BB19 cells bound P. falciparum-infected red blood ce
lls with both the FCR-3 and the ITO4 strains. Antibodies to CD36 and I
CAM-1 partially inhibited the binding of the FCR-3 and the ITO4 lines,
respectively. These findings suggest that BB19 cells may be useful in
the analysis of receptor-based cytoadherence and sequestration, as we
ll as in the cell biology of microvessel formation. Copyright (C) 1996
Australian Society for Parasitology.