Jm. Raleigh et Ent. Meeusen, DEVELOPMENTALLY-REGULATED EXPRESSION OF A HAEMONCHUS-CONTORTUS SURFACE-ANTIGEN, International journal for parasitology, 26(6), 1996, pp. 673-675
All life cycle stages of Haemonchus contortus were screened with antib
ody-secreting cell (ASC) probes generated from immune sheep 5 days aft
er a challenge infection, An antigen with an approximate molecular wei
ght of 70-90 kDa was found to be predominantly expressed in the 3rd-st
age larvae, while a molecule with an approximate molecular weight of 2
8-31 kDa was recognised by the ASC-probes in the earlier developmental
stages, Antisera generated against the purified 70-90 kDa molecule an
d antibodies eluted from the 70-90 kDa region in the 3rd-stage larvae
were found to react with the 28-31 kDa region in the 2nd-stage larvae.
These results suggest that the 70-90 kDa antigen, previously identifi
ed as a major 3rd-stage surface antigen, is synthesised as a lower mol
ecular weight molecule in the earlier larval stages and assembled into
a higher molecular weight complex through non-reducible covalent bond
s. Copyright (C) 1996 Australian Society for Parasitology.