LIPID-PEROXIDATION AFTER ACUTE RENAL ISCHEMIA AND REPERFUSION IN RATS- THE EFFECT OF TRIMETAZIDINE

Lipid peroxidation is a critical pathway of reactive oxygen species in ducing tissue, injury in postischemic acute renal failure. In order to evaluate the effect of renal ischemia reperfusion on kidneys, renal t issue malondialdehyde (MDA, nmol/g wet weight) concentration was measu red in 29 male Wistar rats subjected to a midline abdominal incision a nd 60 min occlusion of the left renal artery. A right nephrectomy was performed at the beginning of the ischemic period. The animals were se parated in four groups. Groups 1 (n = 7) and 3 (n = 7) underwent 60 mi n of ischemia and 15 min of repefusion, respectively Groups 2 (n = 8) and 4 (n = 7) were subjected to the same procedure but, in addition, t hey received 2.5 mg/kg TMZ into the tail vein 2 h prior to the left re nal artery occlusion. A significant elevation of MDA after 60 min of i schemia (1.43 vs. 2.1, p < 0.001), which was augmented after 15 min of repefusion (1.4 vs. 3.72, p < 0.001) was observed. Furthermore, there was a significant reduction of renal tissue MDA in ischemic rats trea ted with TMZ (group 3) (2.1 vs. 1.52, p < 0.001). The maximum reductio n of renal tissue MDA was observed in ischemic-reperfused rats (group 4) that had received TMZ (3.72 vs. 1.36, p < 0.001). It is suggested t hat lipid peroxidation is a critical event in postischemic acute renal failure, and TMZ is a useful protective agent of renal damage from ox ygen free radicals.