COMPARISON OF 2 SUBSTRATES IN DETERMINATION OF PLASMA CHOLINESTERASE ACTIVITY AND GENETIC-VARIABILITY

Two substrates, acetyl thiocholine iodide \ATCI\ and benzoylcholine ch loride \BCC\ were compared for the determination of plasma cholinester ase \ChE\ levels and after incorporation of dibucaine and sodium fluor ide in the assay, their usefulness in determining plasma ChE genetic v ariability was assessed in 64 healthy subjects, With both substrates, plasma ChE levels were found to be in the reference range, However, AT CI could detect only two variants with the usual phenotype \UU\ in 60 of 64 \93.75%\ subjects whereas with BCC 6 different groups could be d etermined, Though both substrates are of equal value in estimation of plasma ChE levels, BCC is definitely superior in determining its genet ic variants