YEAST AND BACTERIAL ARTIFICIAL CHROMOSOME (YAC AND BAC) CLONES OF THEMODEL LEGUME LOTUS-JAPONICUS

Authors
PILLAI SV FUNKE RP GRESSHOFF PM
Citation
Sv. Pillai et al., YEAST AND BACTERIAL ARTIFICIAL CHROMOSOME (YAC AND BAC) CLONES OF THEMODEL LEGUME LOTUS-JAPONICUS, Symbiosis, 21(2), 1996, pp. 149-164
Citations number
33
Categorie Soggetti
Microbiology
Journal title
Symbiosis → ACNP
ISSN journal
03345114
Volume
21
Issue
2
Year of publication
1996
Pages
149 - 164
Database
ISI
SICI code
0334-5114(1996)21:2<149:YABAC(>2.0.ZU;2-3
Abstract
Lotus japonicus is a model legume with a small diploid genome (n=6), s hort life cycle and high transformation ability. As a step towards pos itional cloning of developmental genes in L. japonicus we cloned piece s of high molecular weight (HMW) DNA into yeast and bacterial artifici al chromosomes vectors (YACs and BACs) and transformed them into their respective hosts. Standardized cloning and transformation conditions as well as verification of inserts by pulsed field gradient electropho resis (PFGE) and Southern hybridization are described.