ACCUMULATION OF HSP70 IN JUVENILE AND ADULT RAINBOW-TROUT GILL EXPOSED TO METAL-CONTAMINATED WATER AND OR DIET/

The accumulation of heat shock cognate (hsc)/heat shock protein (hsp) 70 was measured in gill and liver from juvenile and adult rainbow trou t (Oncorhynchus mykiss) exposed to waterborne metals and/or fed a meta l-contaminated diet. These experiments are part of a larger study in w hich physiological responses were documented in adult and juvenile rai nbow trout after exposure to food-borne and/or waterborne metals for 2 1 d. Western blot analyses were used to measure the accumulation of hs p70 and hsc70. Hsc/hsp70 levels were significantly increased in gills of juveniles exposed to metals, in both water and food. The importance of metals in the diet as well as the mater was confirmed for the juve niles. Significant increases in hsp/hsc70 could not be demonstrated in the adults.