PURIFICATION AND CHARACTERIZATION OF GLUCOAMYLASE PRODUCED BY ASPERGILLUS-NIGER IN SOLID-STATE FERMENTATION

Authors
SELVAKUMAR P ASHAKUMARY L HELEN A PANDEY A
Citation
P. Selvakumar et al., PURIFICATION AND CHARACTERIZATION OF GLUCOAMYLASE PRODUCED BY ASPERGILLUS-NIGER IN SOLID-STATE FERMENTATION, Letters in applied microbiology, 23(6), 1996, pp. 403-406
Citations number
13
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
23
Issue
6
Year of publication
1996
Pages
403 - 406
Database
ISI
SICI code
0266-8254(1996)23:6<403:PACOGP>2.0.ZU;2-G
Abstract
Glucoamylases produced by Aspergillus niger grown on wheat bran in sol id cultures were purified. Four different forms, GA I, GA I', GA II an d GA III, were found having apparent molecular weights of 112000, 1040 00, 74000 and 61000 Da respectively. The enzymes are glycoproteins wit h a carbohydrate content of 16%, and optimal activity at 60 degrees C and pH 4.4. Activity was strongly inhibited by Hg2+ while Mn2+ and Fe2 + were stimulatory. The K-m values for the degradation of starch and m altose were 3.5 and 7.8 mg ml(-1), respectively.