S. Kriston et al., SEROPREVALENCE OF ASTROVIRUS TYPE-1 AND TYPE-6 IN LONDON, DETERMINED USING RECOMBINANT VIRUS-ANTIGEN, Epidemiology and infection, 117(1), 1996, pp. 159-164
We have developed a microimmunofluorescence test (IF) which uses cells
infected with a recombinant baculovirus which expresses the capsid pr
oteins of astrovirus types 1 or 6. The IF test was sensitive and speci
fic and the results for human astrovirus type 1 (HAst-1) were comparab
le to those obtained by immune electronmicroscopy and radioimmunoassay
. Application of the test to a panel of 273 sera collected from patien
ts and staff at two childrens hospitals in London showed that over 50%
of the population were infected by HAst-1 between the age of 5 and 12
months rising to 90% by 5 years, whereas human astrovirus type 6 (HAs
t6) was relatively uncommon (10-30%) in all age groups.