DETECTION OF IMMOBILIZED AMPLICONS BY ELISA-LIKE TECHNIQUES

The NucleoLink(TM) surface is a physically modified, thermostable, opt ically clear resin, It allows the covalent binding of 5'-phosphorylate d oligonucleotides. Target DNA amplification by polymerase chain react ion (PCR) is accomplished by asymmetric amplification on the covalentl y immobilized primer that develops into immobilized amplicons, A DNA f ragment of bovine leukemia virus is used as a model system for the det ection of immobilized amplicons by ELISA-like techniques, Covalently b ound oligonucleotides are also utilized as capture probe in the hybrid ization-based signal amplification for detection of an infectious orga nism.