PREPARATION OF A DNA COMPLEX WITH LIPOGLUTAMIDE HAVING TETRAETHYLENE GLYCOL TAILS, AND ITS APPLICATION TO DNA DELIVERY INTO TUMOR-CELLS

We newly prepared a polyion complex of DNA with lipoglutamide, -hydrox y-7,10,13,16-tetraoxaoctadecyl)-L-glutamide hydrochloride (abbreviated to 2EO(4)C(6)N(+)). Formation and characterization of the DNA/2EO(4)C (6)N(+) complex were investigated by elemental analysis, melting tempe rature, and light-scattering measurements. Compaction of DNA by bindin g with the cationic 2EO(4)C(6)N(+) was seen by multi-angle light scatt ering. Aggregation of the DNA/2EO(4)C(6)N(+) complex was significantly depressed compared with the DNA complex without tetraethylene glycol. The DNA/2EO(4)C(6)N(+) complex had low cytotoxicity and efficient int ernalization into tumor cells compared with native DNA.