ALLELIC VARIATIONS OF THE HUMAN HISTAMINE H-2-RECEPTOR GENE

THE gene encoding the human histamine H-2 receptor (H2R) has previousl y been cloned and sequenced from gastric cDNA. Following PCR amplifica tion of a fragment of the H2R gene from total human DNA, three single nucleotide base substitutions were observed and confirmed when compare d with the previously published sequence. One of these base changes in troduces an additional TaqI restriction endonuclease site in the codin g portion of the gene. PCR amplification of human H2R gene fragments f ollowed by cleavage with TaqI demonstrated the existence of allelic va riation of the human H2R gene.