CHARACTERIZATION OF THE HUMAN S100A-12 (CALGRANULIN-C, P6, CAAF1, CGRP) GENE, A NEW MEMBER OF THE S100 GENE-CLUSTER ON CHROMOSOME 1Q21

Here, we report the characterization of a human cDNA coding for the re cently published amino acid sequence of a calcium-binding S100 protein , S100A12 (CGRP, calgranulin C, CAAF1, p6). The exon/intron structure of the S100A12 gene is similar to most other S100 genes. It is compose d of three exons which are divided by two introns of 900 bp and 400 bp . The protein is encoded by sequences in exons 2 and 3, with exon 2 co ding for the N-terminal 45 amino acids and exon 3 coding for the C-ter minal 46 amino acids. So far, ten S100 genes are known to be located o n human chromosome 1q21 in a clustered organization. Hence, we investi gated whether S100A11 (S100C, calgizzarin) and S100A12 are also locali zed in the S100 gene cluster. We found both genes within the cluster, with S100A11 being close to S100A10 and S100A12 between the genes S100 A8 and S100A9. Therefore, the S100 gene cluster now is composed of 12 differentially expressed family members.