HPLC METHOD FOR CYST(E)INE AND METHIONINE IN INFANT FORMULAS

Authors
TORAN AA BARBERA R FARRE R LAGARDA MJ LOPEZ JC
Citation
Aa. Toran et al., HPLC METHOD FOR CYST(E)INE AND METHIONINE IN INFANT FORMULAS, Journal of food science, 61(6), 1996, pp. 1132
Citations number
27
Categorie Soggetti
Food Science & Tenology
Journal title
Journal of food science → ACNP
ISSN journal
00221147
Volume
61
Issue
6
Year of publication
1996
Database
ISI
SICI code
0022-1147(1996)61:6<1132:HMFCAM>2.0.ZU;2-P
Abstract
Cyst(e)ine and methionine were converted into cysteic acid and methion ine sulfone by oxidizing with performic acid. The oxidized samples wer e then subjected to acid hydrolysis (6N HCl, 105-110 degrees C/24 hr). After derivatization with phenylisothiocyanate, reverse phase HPLC se paration was carried out al 48 degrees C and with UV detection. Differ ent gradients and pH values in the eluent were assayed to determine th e best resolution. Analytical parameters, detection and quantification limits, linearity, precision and accuracy, were determined. The metho d was reliable and accurate for measuring cyst(e)ine and methionine in infant formulae.