CLONING OF CHICKEN SLOW MUSCLE TROPONIN-T AND ITS SEQUENCE COMPARISONWITH THAT OF HUMAN

A full-length cDNA coding for chicken slow muscle troponin T (TnT) was for the first time isolated from a cDNA library of 10-day-old embryos , using an RT-PCR product of chicken slow muscle TnT. It showed about 60% homology for chicken fast and slow muscle TnTs and 75.2% for human slow muscle TnT. The 16 amino acid sequence found in the carboxyl ter minus of human slow muscle TnT was absent in the chicken slow muscle T nT. The 5E-5A-7E sequence found in the amino terminal region of chicke n slow muscle TnT was partly similar to the counterpart of human slow muscle TnT, bur not to those of chicken fast and cardiac muscle TnTs. With this report of chicken slow muscle TnT, cDNA information on chick en TnTs of all three striated muscles was completed following those of human TnTs. (C) 1996 Academic Press, Inc.