2 NUCLEAR PROTEINS BIND TO THE PROMOTER P3 REGION OF THE HUMAN PARATHYROID HORMONE-RELATED PEPTIDE GENE

We have used a 5' regulatory sequence fragment of the human parathyroi d hormone-related peptide (PTHrP) gene to identify nuclear DNA-binding proteins (DBP), using South-Western analysis. The PTHrB 549 bp DNA fr agment was amplified from a human genomic DNA, and composed of 5' non- coding exon Ic, the intervening intron and 5' non-coding exon II. The DNA fragment bound very specifically to a 70 kDa and a 65 kD protein f rom the nuclear extract of human hepatocyte, HepG2, and keratinocyte, SVK-14, cell lines. This is the first evidence of a physical binding b etween nuclear protein and the human PTHrP gene. The 70 kDa and 65 kDa nuclear proteins may have a role in the regulation of human PTHrP gen e expression. (C) 1996 Academic Press, Inc.