MULTIPLE EXPRESSION OF LY-6C AND ACCUMULATION OF A LY-6C PRE-MESSENGER-RNA IN ACTIVATED MACROPHAGES INVOLVED IN REJECTION OF AN ALLOGRAFTEDTUMOR

We established a monoclonal antibody against a surface antigen of acti vated macrophages (Mos) involved in the rejection of an allografted mo use tumor. Meth A. Bacteria-elicited Mos also expressed the antigen bu t resident or inflammatory Mos, or other leukocytes, did not. The anti gen was identified as Ly-6C by isolation of cDNA clones encoding it. T wo-dimensional immunoblotting revealed that Ly-6C of thr Mos exists in multiple forms with a similar size but with different isoelectric poi nts. A Ly-6C pre-mRNA also accumulated abundantly in the Mps. Thus, th e multiple expression of Ly-6C and the accumulation of the pre-mRNA ar e the features of allograft- and bacteria-elicited Mos. (C) 1996 Acade mic Press, Inc.