L. Montanari et al., SELECTIVE EXTRACTION OF PHOSPHOLIPID MIXTURES BY SUPERCRITICAL CARBON-DIOXIDE AND COSOLVENTS, Journal of food science, 61(6), 1996, pp. 1230
To optimize and enhance the value of a previously developed supercriti
cal fluids (SF) process for removing oil from soybean flakes, we devis
ed a two-step, sequential scheme for extraction of oil and phospholipi
d-containing fractions using SC-CO2 alone or with ethanol. PLs were se
lectively removed from the flakes using the SC-CO2/ethanol mixture. Ph
osphatidylcholine (PC) and phosphatidylethanolamine (PE) were more rea
dily solubilized in the SC-CO2/cosolvent mixtures than were phosphatid
ylinositol and phosphatidic acid. The extent of recovery of PC and PE
was a function of the molar fraction of cosolvent. Some fractionation
of constituent phospholipids could be achieved by varying the molar fr
action of ethanol. The extracts from the SF process were characterized
by inductively coupled plasma spectroscopy (ICP) and HPLC with evapor
ative light scattering detection.