PHAGE DIABODY REPERTOIRES FOR SELECTION OF LARGE NUMBERS OF BISPECIFIC ANTIBODY FRAGMENTS

Methods for the generation of large numbers of different bispecific an tibodies are presented. Cloning strategies are detailed to create repe rtoires of bispecific diabody molecules with variability on one or bot h of the antigen binding sites. This diabody format, when combined wit h the power of phage display technology, allows the generation and ana lysis of thousands of different bispecific molecules. Selection for bi nding presumably also selects for more stable diabodies. Phage diabody libraries enable screening or selection of the best combination bispe cific molecule with regards to affinity of binding, epitope recognitio n and pairing before manufacture of the best candidate.