Alternaria macrospora Zimm. causing leaf spots on cotton, was grown on
three culture media viz. Czapek-Dox liquid medium (CD), CD with 0.5%
peptone and CD with 0.5% yeast extract for testing its toxigenic poten
tial. The culture filtrates of the pathogen exhibited phytotoxicity ag
ainst cotton (host), tomato and bajra (non-hosts) as well as antibacte
rial activity. The culture filtrates collected from CD with 0.5% pepto
ne were more toxic to the test plants than the other two. The toxic ef
fects of culture filtrates gradually increased with aging of the cultu
res in the three cases. Analysis of the crude toxin revealed the prese
nce of two phytotoxic compounds, of which one was identified as tenuaz
onic acid.