M. Nicolaisen et R. Geisen, TRANSFORMATION OF PENICILLIUM-FREII AND A RAPID PCR SCREENING-PROCEDURE FOR COTRANSFORMATION EVENTS, Microbiological research, 151(3), 1996, pp. 281-284
A strain of Penicillium freii was transformed to hygromycin B resistan
ce using the pAN7-1 plasmid. The transformation frequency was approxim
ately 100 transformants per mu g DNA per 1x10(7) protoplasts. Southern
blotting revealed different types of integration into the genome of P
. freii. When non-selectable plasmid DNA was added together with pAN7-
1, this resulted in a cotransformation frequency of more then 60%. The
cotransformation frequency was estimated using a facile DNA extractio
n method together with PCR.