M. Nicolaisen et al., 2D-PAGE EXAMINATION OF MESSENGER-RNA POPULATIONS FROM PENICILLIUM-FREII MUTANTS DEFICIENT IN XANTHOMEGNIN BIOSYNTHESIS, Microbiological research, 151(3), 1996, pp. 285-290
Penicillium freii (Lund and Frisvad 1994) mutants deficient in the syn
thesis of xanthomegnin were isolated. In vitro translated mRNA populat
ions from selected radiation induced mutants and naturally occurring P
. freii strains not able to produce xanthomegnin were examined by 2-di
mensional polyacrylamide gel electrophoresis (2D-PAGE). Specific trans
lation products were absent in mutants and natural isolates unable to
produce xanthomegnin metabolites. One mutant (TSM 73) did not produce
several of these translation products, indicating that a mutation in a
regulatory gene involved in xanthomegnin production had occcurred.