A seroepidemiological survey, using an indirect immunofluorescence tes
t against Coxiella burnetii (antigenic phase II), was carried out in L
eon province, north-western Spain. A total of 406 serum samples was co
llected from people (from 15 to more than 65 years old) living in a ru
ral environment during the winter and spring of 1994. The overall prev
alence was 40.6%, titres ranged from 1:80 to 1:640, and a titre of 1:8
0 was encountered among 60.6% of positive samples. A significant highe
r prevalence was observed among males globally, as well as among those
aged 15-44 and 45-64 years old; however, no difference was encountere
d among males and females older than 64 years. In the same way, a sign
ificant higher Q fever prevalence was observed among individuals havin
g occupations related to agriculture and among those having close rela
tionship with animals.