A SOBEMOVIRUS FROM DACTYLIS-GLOMERATA WITH LUTEOVIRUS-LIKE ORGANIZATION OF THE REPLICASE AND PROTEASE GENE MODULE

A virus isolated from cocksfoot Dactylis glomerata had the host range, symptoms, and morphology described earlier for the cocksfoot mottle v irus (CfMV). The complete 4083-nt sequence of its genomic RNA was dete rmined (GeneBank LA0905) and found to be most similar to that of the r ecently studied Norwegian CfMV isolate; thus the new virus could be re garded as a Russian strain of CfMV. Four long ORFs encode proteins of 12, 61, 56, and 28K (5' to 3'). At the termini there are untranslated regions of 69 (5') and 228 nt (3'). Analysis of the amino acid sequenc e of 28K revealed high similarity with sobemovirus coat proteins. Sign ificant similarity was also observed for putative RNA-dependent RNA po lymerase and protease; however, the arrangement and the expression mod e of the corresponding genes were analogous to those of luteoviruses. The 5' end of subgenomic RNA was mapped; this region contained the str uctural motif typical of all sobemoviruses.