FATTY-ACIDS SELECTIVELY INHIBIT EUKARYOTIC DNA-POLYMERASE ACTIVITIES IN-VITRO

Authors
MIZUSHINA Y TANAKA N YAGI H KUROSAWA T ONOUE M SETO H HORIE T AOYAGI N YAMAOKA M MATSUKAGE A YOSHIDA S SAKAGUCHI K
Citation
Y. Mizushina et al., FATTY-ACIDS SELECTIVELY INHIBIT EUKARYOTIC DNA-POLYMERASE ACTIVITIES IN-VITRO, Biochimica et biophysica acta, N. Gene structure and expression, 1308(3), 1996, pp. 256-262
Citations number
12
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1308
Issue
3
Year of publication
1996
Pages
256 - 262
Database
ISI
SICI code
0167-4781(1996)1308:3<256:FSIEDA>2.0.ZU;2-R
Abstract
The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activ ities of DNA polymerase alpha and/or beta in vitro. The kinetics of in hibition by linoleic acid showed that DNA polymerase alpha was non-com petitively inhibited with respect to the DNA template and substrate (d TTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.