ISOLATION OF HIGH-MOLECULAR-LENGTH DNA FROM HUMAN SKIN

Authors
BENNETT PV GANGE RW HACHAM H HEJMADI VS MORAN M RAY S SUTHERLAND BM
Citation
Pv. Bennett et al., ISOLATION OF HIGH-MOLECULAR-LENGTH DNA FROM HUMAN SKIN, BioTechniques, 21(3), 1996, pp. 458
Citations number
16
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
21
Issue
3
Year of publication
1996
Database
ISI
SICI code
0736-6205(1996)21:3<458:IOHDFH>2.0.ZU;2-R
Abstract
An agarose plug method for isolating high-molecular-length DNA from ma mmalian tissues has been developed , including from those that are dif ficult, such as skin. It gives high yields of DNA that contain a minim um of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is read ily adaptable to field or clinical studies.