SUBTRACTIVE HYBRIDIZATION, A TECHNIQUE FOR EXTRACTION OF DNA-SEQUENCES DISTINGUISHING 2 CLOSELY-RELATED GENOMES - CRITICAL ANALYSIS

Authors
ERMOLAEVA OD SVERDLOV ED
Citation
Od. Ermolaeva et Ed. Sverdlov, SUBTRACTIVE HYBRIDIZATION, A TECHNIQUE FOR EXTRACTION OF DNA-SEQUENCES DISTINGUISHING 2 CLOSELY-RELATED GENOMES - CRITICAL ANALYSIS, GENET A-BIO, 13(2), 1996, pp. 49-58
Citations number
35
Categorie Soggetti
Genetics & Heredity","Biochemical Research Methods
Journal title
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING → ACNP
ISSN journal
10503862
Volume
13
Issue
2
Year of publication
1996
Pages
49 - 58
Database
ISI
SICI code
1050-3862(1996)13:2<49:SHATFE>2.0.ZU;2-O
Abstract
The present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtraction are discussed. Using the kinetic model of subtractive hybr idization developed by us previously (Sverdlov and Ermolaeva, 1993; Sv erdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique shoul d be further advanced based on subtraction of single-stranded DNAs. Th is strategy would enable one to efficiently extract target sequences o mitting the stage of genome simplification.