Vh. Beaumont et al., COMPARISON OF RAPD AND RFLP MARKERS FOR MAPPING F2 GENERATIONS IN MAIZE (ZEA-MAYS L), Theoretical and Applied Genetics, 93(4), 1996, pp. 606-612
The F-2 generations from two maize crosses were used to compare the ab
ility of RAPD and RFLP marker systems to create a genetic linkage map,
Both RFLPs and RAPDs were shown to provide Mendelian-type markers. Mo
st of the RFLPs (80%) could be placed with a good level of certainty (
LOD > 4) on the genetic linkage map, However, because of their dominan
t nature, only between 37% and 59% of the RAPDs could be placed with s
uch a LOD score, The use of combined data from RFLPs and RAPDs increas
es the level of information provided by RAPDs and allows the creation
of a combined RFLP/RAPD genetic linkage map, Thus, the RAPD technique
was found to be a powerful method to provide improved probes coverage
on a previously created RFLP map and to locate markers linked to chrom
osomal regions of interest.