Mj. Grossel et al., TRANSCRIPTIONAL ACTIVATION FUNCTION IS NOT REQUIRED FOR STIMULATION OF DNA-REPLICATION BY BOVINE PAPILLOMAVIRUS TYPE-1 E2, Journal of virology, 70(10), 1996, pp. 7264-7269
Bovine papillomavirus type 1 replication was previously shown to requi
re both the El initiator protein and the E2 transactivator protein. We
show here that E1, in the absence of E2, is sufficient for low-level
bovine papillomavirus type 1 DNA replication in C-33A cells. In additi
on, studies of genetically isolated E2 point mutants demonstrate that
enhancement of replication by E2 does not require its transcriptional
activation function. The uncoupling of the E2 functions suggests that
stimulation of transcription and replication by enhancer proteins occu
rs via divergent mechanisms.