PROPERTIES OF LOW-DENSITY LIPOPROTEINS RELEVANT TO OXIDATIVE MODIFICATIONS CHANGE PARADOXICALLY DURING AGING

Atherosclerosis is a common problem among the elderly. Because lipid p eroxidation is considered a contributor to the development of atherosc lerosis, we compared oxidative properties of lipoproteins in an otherw ise healthy (SENIEUR-classified) aged population (65-74 years) with yo ung controls (18-30 years). Relative amounts of oxidatively altered lo w density lipoprotein (LDL), estimated by means of an antibody against LDL, modified by 4-hydroxynonenal, a product of lipid peroxidation, w ere increased marginally in serum from the elderly (9.8 vs. 7.4%, P = 0.07), In contrast, isolated LDL from the elderly revealed a decreased susceptibility to in vitro oxidation: the lag time was increased (2.3 4 vs. 2.10 h, P < 0.01), and the maximal rate of LDL oxidation decreas ed (0.88 vs. 1.01 O.D./h, P = 0.001). However, there were no age-relat ed changes in lipid composition of native LDL and consumption of fatty acids-during in vitro oxidation. The serum concentrations of ascorbic acid and most lipophilic anti-oxidants (the latter expressed per g se rum lipids) were significantly decreased in the elderly except tocophe rols which tended to be higher. In conclusion, our data reveal paradox age-related alterations of LDL as to its behaviour in oxidation in vi vo vs. in vitro.