LONG-WAVELENGTH STOPPED-FLOW FLUOROMETRIC-DETERMINATION OF LYSOZYME

The use of Cresyl Violet and sodium dodecyl sulphate for the kinetic f luorimetric determination of lysozyme from dynamic fluorescence measur ements at a long wavelength in the rang near 600 nm was explored. The high initial rate of this system allows analytical measurements to be made within ca. 2 s after the reactants are mixed, by using the stoppe d-how mixing technique, which makes the method applicable to automatic routine analysis. The calibration graph is linear over the range 0.5- 40 mu g ml(-1) lysozyme hydrochloride and the detection limit of 0.19 mu g ml(-1). The precision is less than 2% (relative standard deviatio n). The results obtained by applying the proposed method to the analys is of pharmaceutical samples with no pretreatment shown how readily it can be adapted for routine analyses. Analytical recoveries ranged bet ween 94.0 and 104.0%.