ROOM-TEMPERATURE PHOSPHORIMETRY STUDIES OF SOME ADDICTIVE DRUGS FOLLOWING DANSYL CHLORIDE LABELING

A room-temperature phosphorimetric (RTP) method for the analysis of ba rbital, codeine, morphine and practolol after labelling with dansyl ch loride (DNS-Cl) is described. The drug-DNS derivatives were obtained b y refluxing with drug-ethyl acetate solutions and solid DNS-Cl in the presence of anhydrous potassium carbonate. The reaction conditions wer e investigated in detail. The fluorescence emission of drug-DNS deriva tives shifted to longer wavelengths compared with that of DNS-Cl. The RTP phenomena observed for these derivatives by using a micellar stabi lized room-temperature phosphorescence technique were examined and opt imum conditions for their RTP emission were studied using an orthogona l array design. Derivative RTP spectra were obtained and successfully used to determine practolol by the established method without further separation.