HPLC DETERMINATIONS OF ONDANSETRON WITH SELECTED MEDICATIONS IN 0.9-PERCENT SODIUM-CHLORIDE INJECTION USP

High performance liquid chromatography procedures have been developed for the assay of ondansetron-selected medications in 0.9% sodium chlor ide injection USP. The separation and quantitation of each mixture wer e performed on either an underivatized silica column or a bonded phase column at ambient temperature using either a methanol or acetonitrile -phosphate buffer mobile phase in the 4-5.4 pH ranges with detection s et at 254, 233 or 210 nm. The separations were usually achieved within 20 min. Linearity, limit of detection, retention and tailing factors, resolution, accuracy and precision were calculated for each mixture.