QUANTITATIVE-ANALYSIS OF THE ENANTIOMERIC NAPHTHAQUINONE DERIVATIVES FROM BORAGINACEOUS ROOTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Some Boraginaceous roots contain red colored pigments, alkannin and sh ikonin, which are used in therapy as well as in food and textile indus try. For the quantitative analysis of these enantiomeric naphthaquinon e derivatives, a cellolose carbamate based chiral column (Chiralcel OD ) on HPLC was used by using hexane/2-propanol (90:10) as mobile system . The method was applied to determine the alkannin and shikonin conten ts of the roots of eighteen Boraginaceous plants and results were also compared with those of the LiChrospher RP-18 column.