ANALYSIS OF SODIUM DODECYL SULFATE-STABLE CELL-WALL AMINOPEPTIDASES IN STRAINS OF VIRIDANS STREPTOCOCCI

Authors
LINDER LE LONNIES H SUND ML
Citation
Le. Linder et al., ANALYSIS OF SODIUM DODECYL SULFATE-STABLE CELL-WALL AMINOPEPTIDASES IN STRAINS OF VIRIDANS STREPTOCOCCI, FEMS microbiology letters, 143(1), 1996, pp. 19-23
Citations number
10
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
143
Issue
1
Year of publication
1996
Pages
19 - 23
Database
ISI
SICI code
0378-1097(1996)143:1<19:AOSDSC>2.0.ZU;2-I
Abstract
Cell wall extracts from nine strains of viridans streptococci represen ting five species were analyzed for aminopeptidases. Sodium dodecyl su lfate-polyacrylamide gel electrophoresis in combination with zymogram procedures revealed a common pattern of aminopeptidases in six strains , all having enzyme bands at 89 and 45 kDa. Three strains had an addit ional band at 200 kDa. Crossreactivity between aminopeptidases of all active strains was shown with crossed immunoelectrophoresis. Strains o f Streptococcus mutans and S. sobrinus were without detectable cell wa ll aminopeptidases.