ANALYSIS OF MACROMOLECULAR BRANCHED-CHAIN POLYPEPTIDES BY CAPILLARY ELECTROPHORESIS AND MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Amphoteric poly(Lys-[Glu(1.0)-DL-Ala(4.1)]), (EAK) and anionic poly(Ly s-Ac-Glu(0.98)-DL-Ala(3.98)]), (AcEAK) branched chain polypeptides wer e analyzed by capillary electrophoresis (CE) and micellar elektrokinet ic chromatography (MEKC) in the following buffers. A1: 0.25 N triethyl ammonium phosphate (TEAP) buffer (pH 2.25); A2: 100 mM sodium dodecyl sulfate (SDS) in buffer A1; B1: Na-borate buffer (pH 7.7); B2: 100 mM SDS in buffer B1; C1: Na-borate buffer (pH 11.0); C2: 100 mM SDS in b uffer C1. Both EAK and AcEAK could be separated by a CE mechanism at p H 2.25 and by an MEKC mechanism at pH 11.0. Optimum results were achie ved with CE in buffer A1 and with MEKC in buffer C2.